Lactoferrin Antikörper (Biotin)

Produktnummer ABIN458279

Der Ziege Polyklonal Anti-Lactoferrin-Antikörper wurde für WB, IHC, ELISA und ICC validiert. Er ist geeignet, Lactoferrin in Proben von Affe zu detektieren.

Kurzübersicht für Lactoferrin Antikörper (Biotin) (ABIN458279)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Lactoferrin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-Lactoferrin Antikörper (Biotin)

Spezifität: The antiserum does not cross-react with any other monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail , however a strong cross-reaction with lactoferrin in human milk has been observed.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal goat antiserum to monkey lactoferrin

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized fractions of monkey serum and lactoferrin-depleted monkey milk as required, to eliminate antibodies reacting with other monkey serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from rhesus monkey milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In immunocytochemical and immunohistochemical techniques for the detection of monkey lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:7,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.2. No foreign proteins added.

Buffer: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Details zu Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Andere Bezeichnung: Lactoferrin

Hintergrund: Tested in immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma

Pathways: Transition Metal Ion Homeostasis