Serum Antikörper (FITC)

Produktnummer ABIN458188

Dieser Schwein Polyklonal Antikörper detektiert spezifisch Serum in ELISA, IHC (fro), ICC und IFA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Serum Antikörper (FITC) (ABIN458188)

Target: Serum

Reaktivität: Human

Wirt: Schwein

Klonalität: Polyklonal

Konjugat: FITC

Applikation: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Indirect Immunofluorescence Assay (IFA)

Produktdetails

Verwendungszweck: Swine anti Human Serum proteins, conjugated with FITC

Spezifität: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Swine antiSerum to Human Serum proteins.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Produktmerkmale: In immunoelectrophoresis against normal human serum precipitation can be observed of not less than 15 individual protein components. In direct or indirect fluorescent staining techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Pooled whole human serum of healthy donors and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: FITC-coupled purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Antigendetails

Target: Serum

Andere Bezeichnung: Serum Proteins