TTR Antikörper

Produktnummer ABIN458186

Der Schwein Polyklonal anti-TTR Antikörper wird verwendet zum Nachweis von TTR in Proben von Human. Er wurde validiert für IEP und RID.

Kurzübersicht für TTR Antikörper (ABIN458186)

Target: TTR (Transthyretin (TTR))

Reaktivität: Human

Wirt: Schwein

Klonalität: Polyklonal

Konjugat: Dieser TTR Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Produktdetails anti-TTR Antikörper

Verwendungszweck: Swine anti Human prealbumin

Spezifität: Precipitating polyclonal Swine antiSerum to Human transthyretin (prealbumin).

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.

Produktmerkmale: In immunoelectrophoresis and double radial immunodiffusion against normal human serum the precipitin line obtained shows a reaction of full identity with the purified immunogen. The product does not react with any other proteins component of human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of transthyretin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Aufreinigung: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Transthyretin is the fastest protein in the immunoelectrophoresis pattern of human serum. It consists of four identical polypeptide chins with no carbohydrate. The molecular weight is 55,000 and the concentration in normal serum ranges from 100 to 400 mug/ml. It can bind thyroxine and retinal and its concentration is reduced in severe liver diseases. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Precipitation assays. In immunoelectrophoresis use 2 μL human plasma or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled swine serum. No foreign proteins added.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Details zu TTR

Target: TTR (Transthyretin (TTR))

Andere Bezeichnung: Transthyretin (Prealbumin)

UniProt: P02766

Pathways: Hormone Transport