Inter alpha Trypsin Inhibitor Antikörper

Produktnummer ABIN458167

Dieser Schaf Polyklonal Antikörper detektiert spezifisch Inter alpha Trypsin Inhibitor in IEP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Inter alpha Trypsin Inhibitor Antikörper (ABIN458167)

Target: Inter alpha Trypsin Inhibitor

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunoelectrophoresis (IEP)

Produktdetails

Spezifität: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal sheep antiserum to human inter alpha trypsin inhibitor

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Inter alpha trypsin inhibitor is a proteinase inhibitor with a molecular weight of 160,000. Its mean concentration in human plasma is about 400 µ g/ml. It has been isolated as a homogenous protein for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of inter alpha trypsin inhibitor or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG fraction 13 mg/ml in normal sheep serum diluted 1/2 with PBS. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal human plasma in agar-block immunodiffusion titration.

Buffer: Delipidated, heat inactivated, lyophilized, stable IgG fraction in ½ diluted normal serum.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconsti tute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Antigendetails

Target: Inter alpha Trypsin Inhibitor

Hintergrund: The reactivity of the antiserum is restricted to inter alpha trypsin inhibitor. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen