PF4 Antikörper

Produktnummer ABIN458158

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PF4 in IEP, RID, T und AGE. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PF4 Antikörper (ABIN458158)

Target: PF4 (Platelet Factor 4 (PF4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PF4 Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID), Test (T), Agarose Gel Electrophoresis (AGE)

Produktdetails anti-PF4 Antikörper

Verwendungszweck: Rabbit anti Human platelet factor 4

Spezifität: Precipitating polyclonal Rabbit antiSerum to Human platelet factor 4.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail. In double radial immunodiffusion a reaction has been observed with Bovine, Cat, Dog and Monkey.

Produktmerkmale: The reactivity of the antiserum is restricted to platelet factor 4 as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified immunogen. In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion to identify the presence platelet factor 4. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included. Measurement of platelet factor 4 is of significance for diagnosis of platelet disorders involving platelet alpha granules and to demonstrate increased platelet secretion in vivo. Reduced levels are measured in gray platelet syndrome, whereas elevated levels have been reported in cases as coronary artery disease, myocardial infarction, disseminated intravascular coagulation, cerebrovascular disease and cancer.

Aufreinigung: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Platelet factor 4 is a low molecular weight (MW 7,800) heat stable protein, secreted by alpha granules of the platelets. Purified platelet factor 4 isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion the amount of antiserum required in the agarose gel is usually between 1 and 2 % , depending on the test arrangement. The amount of platelet factor 4 precipitated by 1 mL antiserum is approximately 0.1 mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum Sodium azide. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Details zu PF4

Target: PF4 (Platelet Factor 4 (PF4))

Andere Bezeichnung: Platelet Factor 4

UniProt: P02776