alpha 2 Antiplasmin Antikörper

Produktnummer ABIN458138

Produktdetails anti-alpha 2 Antiplasmin Antikörper

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha 2 Antiplasmin Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP), Immunodiffusion (ID), Radial Immunodiffusion (RID)

Spezifität: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal rabbit antiserum to human alpha-2 antiplasmin

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Alpha-2 antiplasmin is a single-chain glycoprotein with a molecular weight of about 65,000.The normal concentration in human plasma is 60 µ g/ml. Platelets contain significant amounts of the protein, which is released along with platelet factor 4, following thrombin stimulation. It can form 1:1 complexes with circulating plasmin. Alpha-2 antiplasmin is the most important inhibitor of plasmin-mediated fibrinolysis, but also capable of inactivating factor XIIa, kallikrein, factor XIa, thrombin and factor Xa. The importance of the alpha-2 antiplasmin as regulator of the fibrinolytic system was detected by the discovery of a congenital deficiency. Alpha-2 antiplasmin has been isolated from pooled plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). Detection of plasmin-antiplasmin complexes in plasma is evident of plasmin formation and fibrinolysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human plasma in agar-block immuno-diffus

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Details zu alpha 2 Antiplasmin

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Andere Bezeichnung: alpha-2 Antiplasmin (SERPINF2 Produkte)

Synonyme: A2AP antikoerper, AAP antikoerper, ALPHA-2-PI antikoerper, API antikoerper, PLI antikoerper, serpin antikoerper, AI747498 antikoerper, Pli antikoerper, Serpimf2 antikoerper, serpinf2 antikoerper, serpinf2l antikoerper, wu:fd57d08 antikoerper, Dmrs91 antikoerper, Pedf antikoerper, serpin family F member 2 antikoerper, serpin family F member 1 antikoerper, serine (or cysteine) peptidase inhibitor, clade F, member 2 antikoerper, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a antikoerper, SERPINF2 antikoerper, Serpinf2 antikoerper, SERPINF1 antikoerper, serpinf2a antikoerper, Serpinf1 antikoerper

Hintergrund: The defined antibody reactivity is restricted to alpha-2 antiplasmin as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones