F13A1 Antikörper

Produktnummer ABIN458124

Produktdetails anti-F13A1 Antikörper

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser F13A1 Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP), Immunodiffusion (ID)

Spezifität: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal rabbit antiserum to human coagulation factor XIII-A

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Plasma FXIII is an active transglutaminase until it is activated (FXIIIa). It consists of two A subunits joined as a dimer, connected to two B subunits (A2B2). Each A subunit has a molecular weight of 75,000 and each B subunit of 80,000, making an aggregate molecular weight of the whole molecule of 320,000. Normal plasma contains about 20mg/ml. Platelet FXIII consists of only A subunits synthesized in the megakaryocytes. It accounts for about 50% of the total blood FXIII activity with a linear relationship between FXIII level and platelet counts. RAHu/FXIII-A reacts with both plasma and platelet FXIII. Antiserum raised against the B subunit (RAHu/FXIII-S) reacts exclusively with native and activated FXIIIa in plasma. FXIIIa results from activation of FXIII by thrombin after the release of fibrinopeptides A from fibrinogen. Highly purified FXIII-A is used for the immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FXIII. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Increase in plasma FXIII has been observed in type IV hyperlipoproteinaemia and in combined hyperlipodaemia. Congenital and acquired FXIII deficiencies have also been reported. In some cases of myeloma, inhibition of FXIII activity by the paraproteins may occur.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against appropriate concentrations of the antigen in ag

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Details zu F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Andere Bezeichnung: Coagulation Factor XIII-A (F13A1 Produkte)

Synonyme: F13a antikoerper, f13a1 antikoerper, wu:fo83d04 antikoerper, zgc:153334 antikoerper, F13A1 antikoerper, F13A antikoerper, 1200014I03Rik antikoerper, AI462306 antikoerper, coagulation factor XIII A1 chain antikoerper, coagulation factor XIII, A1 polypeptide b antikoerper, coagulation factor XIII A chain antikoerper, coagulation factor XIII, A1 subunit antikoerper, F13a1 antikoerper, f13a1b antikoerper, F13A1 antikoerper

Hintergrund: The defined antibody reactivity is restricted to Factor XIII and FXIIIa in plasma and to platelet FXIII. As tested at the level of sensitivity of immunoprecipitation techniques a single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with A subunits of FXIII. No reaction is obtained with FXIII-depleted plasma and with B subunits