Der Ziege Polyklonal anti-alpha and beta Lipoproteins Antikörper wird verwendet zum Nachweis von alpha and beta Lipoproteins in Proben von Human. Er wurde validiert für IEP.
Kurzübersicht für alpha and beta Lipoproteins Antikörper (ABIN457940)
Target
alpha and beta Lipoproteins
Reaktivität
Human
Wirt
Ziege
Klonalität
Polyklonal
Applikation
Immunoelectrophoresis (IEP)
Spezifität
The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
Produktmerkmale
Precipitating polyclonal goat antiserum to human alpha and beta lipoproteins
Aufreinigung
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Immunogen
Lipoproteins form a heterogeneous system of protein-lipid-carbohydrate complexes which provides highly efficient transport of phospholipids, glycolipids, fatty acids, mono-, di- and triglycerides, cholesterol and cholesterolesters. Concentrations in normal human serum is 230-490 mg/100 ml. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotyp
IgG
Applikationshinweise
In precipitating techniques as immunoelectrophoresis and single or double radial immuno-diffusion to identify the presence of alpha-1lipoprotein in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Konzentration
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:64 when tested against in agar-block immunodiffusion titration against an appropriate
The reactivity of the antiserum is restricted to lipoproteins. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma three precipitin lines are obtained. No reaction is obtained with any other plasma protein component or serum