Der Maus Monoklonal anti-Lactoferrin Antikörper (Klon NI 25) (ABIN457935) detektiert spezifisch Lactoferrin in WB, ELISA und IF.
Dieser Antikörper reagiert spezifisch mit Proben aus Human.
The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Produktmerkmale
Monoclonal mouse antibody to human lactoferrin
Immunogen
Highly purified lactoferrin isolated from human milk.
LTF
Reaktivität: Human
IHC, ELISA, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:100 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Konzentration
IgG concentration is 1.0 mg/ml. No foreign proteins added.
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Target
Lactoferrin (LTF)
(Lactotransferrin (LTF))
Andere Bezeichnung
Lactoferrin
Hintergrund
The reactivity of the antiserum is restricted to lactoferrin as tested in indirect binding immunoblotting and indirect immunoperoxidase staining.