Bence-Jones Protein lambda (Chain lambda), (Free & Bound) Antikörper (Biotin)

Produktnummer ABIN457853

Der Maus Monoklonal anti-Bence-Jones Protein lambda Antikörper wird verwendet zum Nachweis von Bence-Jones Protein lambda in Proben von Human. Er wurde validiert für ELISA und WB.

Kurzübersicht für Bence-Jones Protein lambda (Chain lambda), (Free & Bound) Antikörper (Biotin) (ABIN457853)

Target: Bence-Jones Protein lambda

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB)

Klon: NI 412 and NI 268

Produktdetails

Bindungsspezifität: Chain lambda, Free & Bound

Spezifität: The antibody does not react with any other component of the human immuno-globulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Produktmerkmale: Biotin-conjugated purified monoclonal mouse antibody to human Ig lambda chain, free and bound

Aufreinigung: Purified

Immunogen: Highly purified Bence Jones lambda proteins isolated from pooled human urine.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:100 upwards, in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) is approximately 2.7. No foreign proteins added.

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Antigendetails

Target: Bence-Jones Protein lambda

Andere Bezeichnung: Bence Jones lambda

Hintergrund: The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains, as tested in direct binding enzyme immunoassay, immuno-blotting, immunoprecipitation and indirect immunoperoxidase staining.