Dieser Lactoferrin Antikörper ist konjugiert mit HRP
Applikation
Western Blotting (WB), ELISA, Immunofluorescence (IF)
Spezifität
The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Produktmerkmale
Peroxidase conjugated purified monoclonal mouse antibody to human lactoferrin
Aufreinigung
Purified
Immunogen
Highly purified lactoferrin isolated from human milk.
To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immuno-peroxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:100 upwards, in Western blotting from 1:250 upwards. These data should be interpreted as general recommendations only.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Konzentration
IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
Buffer
Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by addin g 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.