Lactoferrin Antikörper (Biotin)

Produktnummer ABIN457841

Der Maus Monoklonal Anti-Lactoferrin-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, Lactoferrin in Proben von Human zu detektieren.

Kurzübersicht für Lactoferrin Antikörper (Biotin) (ABIN457841)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Lactoferrin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Klon: NI 25

Produktdetails anti-Lactoferrin Antikörper (Biotin)

Spezifität: The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Produktmerkmale: Biotin-conjugated purified monoclonal mouse antibody to human lactoferrin

Aufreinigung: Purified

Immunogen: Highly purified lactoferrin isolated from human milk.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.2. No foreign proteins added.

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by addin g 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Details zu Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Andere Bezeichnung: Lactoferrin

Hintergrund: The reactivity of the antiserum is restricted to lactoferrin as tested in indirect binding immunoblotting and indirect immunoperoxidase staining.

Pathways: Transition Metal Ion Homeostasis