Transferrin Antikörper

Produktnummer ABIN457688

Der Ziege Polyklonal Anti-Transferrin-Antikörper wurde für ELISA, ID, IEP, Neph und RID validiert. Er ist geeignet, Transferrin in Proben von Ente zu detektieren.

Kurzübersicht für Transferrin Antikörper (ABIN457688)

Target: Transferrin (TF)

Reaktivität: Ente

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Transferrin Antikörper ist unkonjugiert

Applikation: ELISA, Immunodiffusion (ID), Immunoelectrophoresis (IEP), Nephelometry (Neph), Radial Immunodiffusion (RID)

Produktdetails anti-Transferrin Antikörper

Spezifität: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however in immunoelectrophoresis a reaction has been observed with chicken and pigeon.

Produktmerkmale: Precipitating polyclonal goat antiserum to duck transferrin

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Purified transferrin isolated from pooled duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence transferrin in duck serum or other body fluids and to determine its concentration. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal duck serum in agar-block immunodiffusion titratio

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refro zen, and preferably used the same day.

Details zu Transferrin

Target: Transferrin (TF)

Andere Bezeichnung: Transferrin

Hintergrund: The reactivity of the antiserum is restricted to transferrin as tested in immunoelectro-phoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin

Pathways: Transition Metal Ion Homeostasis