Fibrinogen Antikörper (FITC)

Produktnummer ABIN457655

Der Ziege Polyklonal anti-Fibrinogen Antikörper wird verwendet zum Nachweis von Fibrinogen in Proben von Hund. Er wurde validiert für ELISA, IHC (fro), ICC und IFA.

Kurzübersicht für Fibrinogen Antikörper (FITC) (ABIN457655)

Target: Fibrinogen

Reaktivität: Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Fibrinogen Antikörper ist konjugiert mit FITC

Applikation: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Indirect Immunofluorescence Assay (IFA)

Produktdetails anti-Fibrinogen Antikörper (FITC)

Verwendungszweck: Goat anti Dog fibrinogen, conjugated with FITC

Spezifität: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Dog fibrinogen

Kreuzreaktivität (Details): The antiSerum does not cross-react with any other component of Dog plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Produktmerkmale: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As reagent for the direct detection of fibrinogen in dog cells, tissues and body fluids in immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C.

Details zu Fibrinogen

Target: Fibrinogen

UniProt: P68213, P02677, P12800