Claudin 1 Antikörper (C-Term)

Produktnummer ABIN457476

Der Kaninchen Polyklonal anti-Claudin 1 Antikörper (ABIN457476) detektiert spezifisch Claudin 1 in WB, IHC, ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund und Affe.

Kurzübersicht für Claudin 1 Antikörper (C-Term) (ABIN457476)

Target: Claudin 1 (CLDN1)

Reaktivität: Human, Maus, Ratte, Hund, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Claudin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Claudin 1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes Claudin 1. Species cross-reactivity: Human, Monkey, Mouse, Canine, and Rat.

Aufreinigung: Immunoaffinity purified

Immunogen: A synthetic peptide derived from the C-terminus of human Claudin 1.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2-4 μg/mL for 2 hrs at RT. Positive control: T47D cells, Skin or Breast carcinoma.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM PBS,  pH 7.6, 0.2 % BSA, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Claudin 1

Target: Claudin 1 (CLDN1)

Andere Bezeichnung: CLDN1 / Claudin 1

Hintergrund: Name/Gene ID: CLDN1
Family: Claudin

Synonyms: CLDN1, Claudin-1, Claudin 1, ILVASC, SEMP1, CLD1

Gen-ID: 9076

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C