Dieses Anti-CD99-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD99 in FACS. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.
The antibody MEM-131 reacts with CD99R, an extracellular epitope restricted to a subset of CD99 Molecule expressed on myeloid cells, NK cells and T lymphocytes.
Kreuzreaktivität (Details)
Human
Aufreinigung
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Immunogen
HPB-ALL human peripheral blood leukemia T-cell line
CD99
Reaktivität: Human
FACS, WB, IHC (p), IP
Wirt: Maus
Monoclonal
MEM-131
unconjugated
Applikationshinweise
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
No reconstitution is necessary.
Buffer
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Do not freeze. Avoid prolonged exposure to light.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).
Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, (1995) (PubMed).
Target
CD99
Andere Bezeichnung
CD99R
Hintergrund
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.,MIC2, HBA71, blood group Xg