RNF34 Antikörper (N-Term)

Produktnummer ABIN453991

Dieser Ziege Polyklonal Antikörper detektiert spezifisch RNF34 in IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh) und Hund.

Kurzübersicht für RNF34 Antikörper (N-Term) (ABIN453991)

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser RNF34 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-RNF34 Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody is expected to recognize both reported isoforms of Human RNF34 (NP_919247.1 and NP_079402.2).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KAGATSMWASCC, from the N Terminus of the protein sequence according to NP_919247.1, NP_079402.2. Genename: RNF34

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/32000. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Kidney shows strongstaining of cytoplasm in DCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Andere Bezeichnung: RFI / RNF34

Hintergrund: RNF34, also known as RING finger protein 34 is a novel modulator of NF-kappaB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein.Synonyms: CARP-1, CARP1, Caspases-8 and -10-associated RING finger protein 1, E3 ubiquitin-protein ligase RNF34, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RIFF

Gen-ID: 80196

NCBI Accession: NP_079402

UniProt: Q969K3