CYB5R3 Antikörper (C-Term)

Produktnummer ABIN453978

Zitiert in 3+ Publikationen. Der Ziege Polyklonal anti-CYB5R3 Antikörper (ABIN453978) detektiert spezifisch CYB5R3 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte, Maus, Hund und Rind (Kuh).

Kurzübersicht für CYB5R3 Antikörper (C-Term) (ABIN453978)

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reaktivität: Ratte, Maus, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CYB5R3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CYB5R3 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody recognizes CYB5R3.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog) and Cow.
Species reactivity (tested):Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PNLERVGHPKERC, from the C-Terminus of the protein sequence according to NP_084063.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection limit dilution 1/32000. Western blot: 0.1-1 μg/mL. Approx 35 kDa band observed in Mouse Liver lysates as well as inMouse and Rat brain lysates (calculated MW of 34.5 kDa according to Mouse NP_084063.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Mouse. Expected from sequence similarity: Rat, Canine (Dog) and Cow.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-CYB5R3 Antikörper (ABIN453978)

Reed, Jarvis, Phillips, Arlt: "Enhanced DNA adduct formation by benzo[a]pyrene in human liver cells lacking cytochrome P450 oxidoreductase." in: Mutation research, Vol. 852, pp. 503162, (2020) (PubMed).

Reed, Indra, Mrizova, Moserova, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine." in: Toxicology and applied pharmacology, Vol. 366, pp. 64-74, (2019) (PubMed).

Reed, Mrizova, Barta, Indra, Moserova, Kopka, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice." in: Archives of toxicology, Vol. 92, Issue 4, pp. 1625-1638, (2019) (PubMed).

Details zu CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Andere Bezeichnung: CYB5R3

Hintergrund: CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and contains 1 FAD-binding FR-type domain. There are two isoforms, NADH-cytochrome b5 reductase 3 membrane bound form and NADH-cytochrome b5 reductase 3 soluble form. Its functions include desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1) in which the enzyme is only deficient in erythrocytes with a mild cyanosis, type 2 (HM2), in which the enzyme is completely deficient, type 3 (HM3) where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied with mental retardation and neurological impairment.Synonyms: B5R, DIA1, Diaphorase-1, NADH-cytochrome b5 reductase 3

Gen-ID: 1727

NCBI Accession: NP_000389

UniProt: P00387

Pathways: SARS-CoV-2 Protein Interaktom