SDHAF1 Antikörper (C-Term)

Produktnummer ABIN452676

Der Ziege Polyklonal anti-SDHAF1 Antikörper (ABIN452676) detektiert spezifisch SDHAF1 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Hund.

Kurzübersicht für SDHAF1 Antikörper (C-Term) (ABIN452676)

Target: SDHAF1 (Succinate Dehydrogenase Complex Assembly Factor 1 (SDHAF1))

Reaktivität: Human, Maus, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SDHAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-SDHAF1 Antikörper

Bindungsspezifität: C-Term

Sequenz: c-HDSTGAPETR PDGR

Spezifität: This antibody detects Human SDHAF1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Canine (Dog).
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_001036096.1. Genename: RNF31

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection limit dilution 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 19 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 12.9 kDa according to NP_001036096.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu SDHAF1

Target: SDHAF1 (Succinate Dehydrogenase Complex Assembly Factor 1 (SDHAF1))

Andere Bezeichnung: SDHAF1

Hintergrund: The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). SDHAF1 Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II (Probable)Synonyms: LYR motif-containing protein 8, SDH assembly factor 1, Succinate dehydrogenase assembly factor 1 mitochondrial

Gen-ID: 644096

NCBI Accession: NP_001036096

UniProt: A6NFY7