EIF5A Antikörper (Internal Region, N-Term)

Produktnummer ABIN452675

Der Ziege Polyklonal anti-EIF5A Antikörper wird verwendet zum Nachweis von EIF5A in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für EIF5A Antikörper (Internal Region, N-Term) (ABIN452675)

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EIF5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-EIF5A Antikörper

Bindungsspezifität: Internal Region, N-Term

Spezifität: Recognizes eIF5A.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of Human eIF5A.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: eIF5A Antibody Detection limit dilution 1/128000. Western blot: 1-3 μg/mL. Approx 20 kDa band observed in MOLT4 lysates (calculated MW of20.0 kDa according to NP_001137232.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Andere Bezeichnung: EIF5A1

Hintergrund: Eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein, the only protein known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. eIF5A protein was previously reported to be involved in the first step of peptide bond formation in translation, however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.Synonyms: Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A-1, Rev-binding factor, eIF-4D, eIF-5A, eIF-5A-1, eIF-5A1

Gen-ID: 1984

NCBI Accession: NP_001137232

UniProt: P63241

Pathways: Regulation of Muscle Cell Differentiation