HSF2 Antikörper

Produktnummer ABIN452666

Der Ratte Monoklonal Anti-HSF2-Antikörper wurde für WB, IF, IP, IHC (fro), EIA und EMSA validiert. Er ist geeignet, HSF2 in Proben von Diverse Spezies zu detektieren.

Kurzübersicht für HSF2 Antikörper (ABIN452666)

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Reaktivität: Diverse Spezies

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Klon: 3-00E-002

Produktdetails anti-HSF2 Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig.

Produktmerkmale: Synonyms: HSF-2, HSTF2, Heat shock transcription factor 2

Aufreinigung: Purified

Immunogen: Purified recombinant mouse SF2 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 4 μg/mL was sufficient for detection of HSF2 of HeLa lysate. Gel Shift Assay (EMSA). Immunoprecipitation. Immunoflourescence. Immunohistochemistry on frozen sections. ELISA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu HSF2

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Andere Bezeichnung: Heat Shock Factor 2 / HSF2

Hintergrund: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).Synonyms: HSF-2, HSTF2, Heat shock transcription factor 2

UniProt: P38533