Calretinin Antikörper

Produktnummer ABIN452385

Dieses Anti-Calretinin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Calretinin in WB, IHC (p) und IHC (fro). Geeignet für Human und Ratte.

Kurzübersicht für Calretinin Antikörper (ABIN452385)

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Calretinin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 6B3

Produktdetails anti-Calretinin Antikörper

Spezifität: Specific for Calretinin. No cross-reaction with Calbindin and Calmodulin. The antibody is suitable for the detection of Calretinin in neurons and may be used as an aid in differential diagnosis of pleura-mesothelioma. This marker is frequently expressed in epitheloid mesothelioma, while occasionally it turns positive in sarcomatous mesothelioma and carcinoma. It was found to be negative in sarcoma (2,3) and in 90 % of adenocarcinoma.

Kreuzreaktivität (Details): Species reactivity (tested):Human and Rat.

Produktmerkmale: Synonyms: CALB2, CAB29, 29 kDa calbindin

Immunogen: Recombinant Human Calretinin 22kDa.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen and Paraffin Sections. (Improvement of staining withUnmasking Fluid G or Unmasking Fluid C)Immunoblotting. (1/40-1/50)Positive Control: Cerebral cortex, pleural mesothelioma. Incubation Time: 60 min at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Stabilized Phosphate buffer, pH 7.3 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Calretinin

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Andere Bezeichnung: Calretinin

Hintergrund: Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98 % sequence homology and 91 % homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. The antibodies against calretinin also labels the mossy fibers throughout, and climbing fibers in a distinct group of folia. They also stain several cell types in the molecular layer. There is no appreciable immunostaining of calretinin in cerebellar cortex. In the rat, antibodies against calretinin stain the Lugaro cells, and some granule cells in lobe X, they also give weak staining of all the granule cells in the other lobes. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.Synonyms: 29 kDa calbindin, CAB29, CALB2

Gen-ID: 794

UniProt: P22676