PDIA3 Antikörper

Produktnummer ABIN452267

Der Maus Monoklonal anti-PDIA3 Antikörper wird verwendet zum Nachweis von PDIA3 in Proben von Human, Maus, Ratte, Hund, Meerschweinchen, Affe, Rind (Kuh), Kaninchen, Schwein und Hamster. Er wurde validiert für WB, IP, IHC (p) und IHC (fro).

Kurzübersicht für PDIA3 Antikörper (ABIN452267)

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Affe, Rind (Kuh), Kaninchen, Schwein, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDIA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MaP-Erp57

Produktdetails anti-PDIA3 Antikörper

Spezifität: This antibody detects ERp57 / GRP58 at ~57 kDa.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit, Rat

Produktmerkmale: Synonyms: Protein disulfide-isomerase A3, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, ERmarker, Disulfide isomerase ER-60

Aufreinigung: Protein G Chromatography

Immunogen: Human recombinant ERp57 (GRP58)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot (8): 1: 2000 (ECL). Immunoprecipitation (9). Immunohistochemistry on frozen sections (8).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Andere Bezeichnung: PDIA3

Hintergrund: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).Synonyms: Disulfide isomerase ER-60, ER marker, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, Protein disulfide-isomerase A3

Gen-ID: 2923

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis