Epidermal Keratin (AA 266-299) Antikörper

Produktnummer ABIN4219252

Dieser Maus Monoklonal Antikörper detektiert spezifisch Epidermal Keratin in IHC (fp), IHC (fro) und WB. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 7+ Publikationen zitiert.

Kurzübersicht für Epidermal Keratin (AA 266-299) Antikörper (ABIN4219252)

Target: Epidermal Keratin

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Klon: AE3

Produktdetails

Bindungsspezifität: AA 266-299

Spezifität: Most basic (type II) keratins

Kreuzreaktivität: Rind (Kuh), Huhn, Human, Ratte (Rattus), Maus, Kaninchen, Affe

Aufreinigung: Ion-exchange chromatography

Immunogen: Human epidermal keratin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: IHC: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: reconstitue with 1 mL deionized water

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodum azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C

Referenzen

Waseem, Karsten, Leigh, Purkis, Waseem, Lane: "Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability." in: Biochemistry, Vol. 43, Issue 5, pp. 1283-95, (2004) (PubMed).

Chesa, Rettig, Melamed: "Expression of cytokeratins in normal and neoplastic colonic epithelial cells. Implications for cellular differentiation and carcinogenesis." in: The American journal of surgical pathology, Vol. 10, Issue 12, pp. 829-35, (1986) (PubMed).

Nomori, Kameya, Shimosato, Saito, Ebihara, Ono: "Nasopharyngeal carcinoma: immunohistochemical study for keratin, secretory component and leukocyte common antigen." in: Japanese journal of clinical oncology, Vol. 15, Issue 1, pp. 95-105, (1985) (PubMed).

Weiss, Eichner, Sun: "Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes." in: The Journal of cell biology, Vol. 98, Issue 4, pp. 1397-406, (1984) (PubMed).

Battifora, Sheibani, Tubbs, Kopinski, Sun: "Antikeratin antibodies in tumor diagnosis. Distinction between seminoma and embryonal carcinoma." in: Cancer, Vol. 54, Issue 5, pp. 843-8, (1984) (PubMed).

Woodcock-Mitchell, Eichner, Nelson, Sun: "Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies." in: The Journal of cell biology, Vol. 95, Issue 2 Pt 1, pp. 580-8, (1983) (PubMed).

Tseng, Jarvinen, Nelson, Huang, Woodcock-Mitchell, Sun: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, (1983) (PubMed).

Antigendetails

Target: Epidermal Keratin

Hintergrund: AE 3 represents an excellent marker fort distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelia and their neoplasms.

Molekulargewicht: 53 - 67 kDa