CD20 Antikörper (C-Term)

Produktnummer ABIN401667

Zitiert in 1 Publikation. Der Ziege Polyklonal anti-CD20 Antikörper (ABIN401667) detektiert spezifisch CD20 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD20 Antikörper (C-Term) (ABIN401667)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CD20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CD20 Antikörper

Bindungsspezifität: C-Term

Sequenz: CQDQESSPIE NDSSP

Spezifität: This antibody recognizes CD20/MS4A1.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_068769.2, NP_690605.1. Genename: MS4A1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western Blot: 3-5 μg/mL at Overnight incubation. Approx 37 kDa band seen in Daudi celllysates. Immunohistochemistry on Paraffin Sections: 2-5 μg/mL. In paraffin embedded Human Spleen, this antibody shows strong membranous staining inthe the follicles formed by B-cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preserative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-CD20 Antikörper (ABIN401667)

Vargas-Inchaustegui, Demers, Shaw, Kang, Ball, Tuero, Musich, Mohanram, Demberg, Karpova, Li, Robert-Guroff: "Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 4, pp. 1700-10, (2016) (PubMed).

Details zu CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Andere Bezeichnung: CD20

Hintergrund: CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 Molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.Synonyms: B-cell marker, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1

Gen-ID: 931

NCBI Accession: NP_068769

UniProt: P11836