SUMF1 Antikörper (Internal Region)

Produktnummer ABIN401525

Der Ziege-Polyklonal Anti-SUMF1-Antikörper wurde für WB und EIA validiert. Er ist geeignet zum Nachweis von SUMF1 in Proben aus Human, Maus, Ratte, Rind (Kuh) und Hund. }

Kurzübersicht für SUMF1 Antikörper (Internal Region) (ABIN401525)

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SUMF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-SUMF1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: c-ETLNPKGPPS GKDR

Spezifität: This antibody detects SUMF1 / FGE (Internal).

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Dog, CowSpecies reactivity (tested):Human, Mouse

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_877437.2 . Genename: SUMF1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 37-40 kDa band observed in Mouse Pancreas, Mouse Eyeand Human Kidney lysates (calculated MW of 40.0 kDa according to NP_877437.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu SUMF1

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Andere Bezeichnung: SUMF1 / FGE

Hintergrund: SUMF1 is a sulfatase modifying enzyme that oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD). MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive.Synonyms: C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

Gen-ID: 285362

NCBI Accession: NP_877437

UniProt: Q8NBK3