PRMT5 Antikörper (Internal Region)

Produktnummer ABIN401522

Der Ziege-Polyklonal Anti-PRMT5-Antikörper wurde für WB und EIA validiert. Er ist geeignet zum Nachweis von PRMT5 in Proben aus Human, Maus, Ratte, Hund, Rind (Kuh) und Schwein. }

Kurzübersicht für PRMT5 Antikörper (Internal Region) (ABIN401522)

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PRMT5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-PRMT5 Antikörper

Bindungsspezifität: Internal Region

Sequenz: RACREKDRDP EAQ

Spezifität: This antibody detects PRMT5 (Internal). It is expected to recognize both isoforms (NP_006100.2, NP_001034708.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Pig, Dog, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_006100.2, NP_001034708.1. Genename: PRMT5

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 70 kDa band observed in Jurkat lysates (calculated MW of72.0 kDa and 71.0 kDa according to NP_006100.2, NP_001034708.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu PRMT5

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Andere Bezeichnung: PRMT5

Hintergrund: Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.Synonyms: 72 kDa ICln-binding protein, HRMT1L5, Histone-arginine N-methyltransferase PRMT5, IBP72, JBP1, Jak-binding protein 1, Protein Arginine N-Methyltransferase 5, SKB1, SKB1Hs

Gen-ID: 10419

NCBI Accession: NP_001034708

UniProt: O14744

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development