Molar Ratio: 10-20 BAC molecules per Goat F(ab')2 Molecule
Aufreinigung
Immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 2,000 - 1: 10,000. Immunohistochemistry: 1: 1,000 - 1: 5,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1.0 mL of deionized water (or equivalent).
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.