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FITC Antikörper (Biotin)

Dieser Anti-FITC Antikörper ist ein Ziege Polyklonal Antikörper zur Detektion von FITC in WB, IHC (fro) und EIA. Geeignet für .
Produktnummer ABIN401490

Kurzübersicht für FITC Antikörper (Biotin) (ABIN401490)

Target

FITC (Fluorescein Isothiocyanate (FITC))

Reaktivität

Bitte anfragen

Wirt

  • 43
  • 18
  • 17
  • 11
  • 8
Ziege

Klonalität

  • 54
  • 42
Polyklonal

Konjugat

  • 34
  • 15
  • 13
  • 12
  • 7
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser FITC Antikörper ist konjugiert mit Biotin

Applikation

  • 59
  • 38
  • 32
  • 18
  • 17
  • 15
  • 14
  • 8
  • 7
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
  • Produktmerkmale

    Molar Ratio: 10-20 BAC molecules per Goat F(ab')2 Molecule

    Aufreinigung

    Immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

    Immunogen

    Fluorescein conjugated to Goat IgG

    Isotyp

    IgG
  • Applikationshinweise

    ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 2,000 - 1: 10,000. Immunohistochemistry: 1: 1,000 - 1: 5,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Restore with 1.0 mL of deionized water (or equivalent).

    Konzentration

    1.0 mg/mL (by UV absorbance at 280 nm)

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
  • Target

    FITC (Fluorescein Isothiocyanate (FITC))

    Andere Bezeichnung

    FITC

    Substanzklasse

    Chemical

    Hintergrund

    Synonyms: Fluorescein Isothiocyanate
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