SAE1 Antikörper (Ser185)

Produktnummer ABIN401467

Der Kaninchen Polyklonal Anti-SAE1-Antikörper wurde für WB und EIA validiert. Er ist geeignet, SAE1 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund und Schimpanse zu detektieren.

Kurzübersicht für SAE1 Antikörper (Ser185) (ABIN401467)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SAE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-SAE1 Antikörper

Bindungsspezifität: Ser185

Spezifität: This antibody is directed against SUMO Activating Enzyme E1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/100,000 - 1/125,000. Western Blot: 1/750 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.71 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Andere Bezeichnung: SAE1 / AOS1

Hintergrund: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A

Gen-ID: 10055

NCBI Accession: NP_005491

UniProt: Q9UBE0