GPX4 Antikörper (C-Term)

Produktnummer ABIN401375

Der Kaninchen Polyklonal anti-GPX4 Antikörper wird verwendet zum Nachweis von GPX4 in Proben von Human, Maus, Ratte und Meerschweinchen. Er wurde validiert für WB und EIA.

Kurzübersicht für GPX4 Antikörper (C-Term) (ABIN401375)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reaktivität: Human, Maus, Ratte, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPX4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-GPX4 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody is directed against GPx4 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Guinea Pig, Human.
Species reactivity (tested):Mouse.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/7,000 - 1/30,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.15 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Andere Bezeichnung: Glutathione Peroxidase 4 / GPX4

Hintergrund: Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions as an antioxidant and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation. GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress. GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters. GPx4 is the only GPx to use phospholipid hydroperoxides as substrates. It can also reduce thymine hydroperoxide. In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione. GPx4 is expressed in high levels in testes, suggesting a role in male fertility. It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.Synonyms: GPx-4, Phospholipid hydroperoxide glutathione peroxidase mitochondrial

Gen-ID: 2879

NCBI Accession: NP_001034936

UniProt: P36969