NOXO1 Antikörper (AA 238-252)

Produktnummer ABIN401322

Dieses Anti-NOXO1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NOXO1 in WB, IHC (p) und EIA. Geeignet für Human, Schimpanse, Hund, Meerschweinchen und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für NOXO1 Antikörper (AA 238-252) (ABIN401322)

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Reaktivität: Human, Schimpanse, Hund, Meerschweinchen, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOXO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-NOXO1 Antikörper

Bindungsspezifität: AA 238-252

Spezifität: This antibody is directed against NOXO1 protein. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from Human and Chimpanzee (100 % homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from Dog and Guinea Pig (87 %), as well as Rat (75 %) and Mouse (68 %). Reactivity against homologues from other sources is not known.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Recombinant protein corresponding to amino acids 238-252 of Human NOXO1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody is tested for use in ELISA, Immunohistochemistry andWestern blot. Recommended Dilutions: ELISA: 1/2,0000-1/100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7. containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.

Referenzen für anti-NOXO1 Antikörper (ABIN401322)

Madrigal-Matute, Fernandez-Garcia, Gomez-Guerrero, Lopez-Franco, Muñoz-Garcia, Egido, Blanco-Colio, Martin-Ventura: "HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis." in: Cardiovascular research, Vol. 95, Issue 1, pp. 116-23, (2012) (PubMed).

Details zu NOXO1

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Andere Bezeichnung: NOXO1

Hintergrund: The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells. NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region. NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5

Gen-ID: 124056

NCBI Accession: NP_653204

UniProt: Q8NFA2