MARK2 Antikörper (Isoform A, pThr595)

Produktnummer ABIN401318

Der Kaninchen Polyklonal anti-MARK2 Antikörper wird verwendet zum Nachweis von MARK2 in Proben von Human, Maus, Ratte, Rind (Kuh) und Hund. Er wurde validiert für WB und EIA.

Kurzübersicht für MARK2 Antikörper (Isoform A, pThr595) (ABIN401318)

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MARK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-MARK2 Antikörper

Bindungsspezifität: Isoform A, pThr595

Spezifität: This antibody is directed against the phosphorylated form of MARK2 at thepT595 residue.

Kreuzreaktivität (Details): Species reactivity (expected):Canine, Bovine, Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000 - 1/3,000. Western Blot: 1/250 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu MARK2

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Andere Bezeichnung: MARK2

Hintergrund: This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.Synonyms: 2, ELKL motif kinase, EMK1, MAP/microtubule affinity-regulating kinase, MARK2, PAR1 homolog, Serine/threonine-protein kinase

Gen-ID: 2011

NCBI Accession: NP_001034558

UniProt: Q7KZI7

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion