ATAD5 Antikörper (AA 63-76)

Produktnummer ABIN401276

Der Kaninchen Polyklonal Anti-ATAD5-Antikörper wurde für WB und EIA validiert. Er ist geeignet, ATAD5 in Proben von Human zu detektieren.

Kurzübersicht für ATAD5 Antikörper (AA 63-76) (ABIN401276)

Target: ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATAD5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ATAD5 Antikörper

Bindungsspezifität: AA 63-76

Spezifität: This antibody is directed against Elg1 protein.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 3,000 - 1: 15,000. Western blot: 1: 500 - 1: 2,000 (Expect a band approximately 120 kDa in size correspondingto Elg1 in the appropriate cell lysate or extract).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.50 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu ATAD5

Target: ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))

Andere Bezeichnung: ATAD5

Hintergrund: ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120 kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207 kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.Synonyms: ATPase family AAA domain-containing protein 5, C17orf41, Chromosome fragility-associated gene 1 protein, FRAG1

Gen-ID: 79915

NCBI Accession: NP_079133

UniProt: Q96QE3