NCAPH Antikörper (AA 440-457)

Produktnummer ABIN401274

Der Kaninchen Polyklonal anti-NCAPH Antikörper (ABIN401274) detektiert spezifisch NCAPH in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Schimpanse.

Kurzübersicht für NCAPH Antikörper (AA 440-457) (ABIN401274)

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Reaktivität: Human, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCAPH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-NCAPH Antikörper

Bindungsspezifität: AA 440-457

Spezifität: This antibody reacts against BRRN1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, Mouse, Rat, Canine, Chicken.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 440-457 of Human BRRN-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/8000 - 1/35000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.93 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu NCAPH

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Andere Bezeichnung: NCAPH

Hintergrund: BRNN1, also called Barren, Condensin subunit 2, Barren homolog protein 1, Chromosomeassociated protein H, hCAP-H, and XCAP-H homolog, is encoded by a gene that is a member of the barr gene family. Brnn1 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex contains the SMC2L1 and SMC4L1 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. BRRN1 has both a nuclear and cytoplasmic localization. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2 leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. BRRN1 is widely expressed at low level in most proliferating cells. CDC2 phosphorylates BRRN1. Its phosphorylation, as well as that of CNAP1 and CAPG subunits, activates the condensin complex and is required for chromosome condensation.Synonyms: BRRN, BRRN1, CAPH, Chromosome-associated protein H, KIAA0074

Gen-ID: 23397

NCBI Accession: NP_056156

UniProt: Q15003