Der Kaninchen Polyklonal anti-Transferrin Antikörper (ABIN401187) detektiert spezifisch Transferrin in WB, EIA und IHC (fro).
Dieser Antikörper reagiert spezifisch mit Proben aus Human.
Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 µm filtered, aseptically filled, and stored at -20°C. An aliquot was submitted for final qualification.
ELISA: 1: 12,000 to 1: 43,000, 1.0 μg of Human Transferrin in a standard sandwich ELISAusing Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L] and TMB as asubstrate for 30 minutes at room temperature. Western blot. Immunohistochemistry in frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.