KLH Antikörper (Alkaline Phosphatase (AP))
Kurzübersicht für KLH Antikörper (Alkaline Phosphatase (AP)) (ABIN401179)
Target
Alle KLH Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Aufreinigung
- Affinity chromatography
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Immunogen
- KLH (Keyhole Limpet Hemocyanin)
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Isotyp
- IgG
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Applikationshinweise
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Western BLot. ELISA: 1/3,000 - 1/13,500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
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Buffer
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL Bovine Serum Albumin (BSA) and 0.01 % (w/v) Sodium Azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- DO NOT FREEZE!!
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C.
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- KLH (Keyhole Limpet Hemocyanin (KLH))
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Hintergrund
- KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.Synonyms: Keyhole Limpet Hemocyanine
Target
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