ATPase (Na/K) Antikörper

Produktnummer ABIN401097

Der Huhn Polyklonal anti-ATPase (Na/K) Antikörper (ABIN401097) detektiert spezifisch ATPase (Na/K) in WB, IHC (fro) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Hund.

Kurzübersicht für ATPase (Na/K) Antikörper (ABIN401097)

Target: ATPase (Na/K)

Reaktivität: Hund

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser ATPase (Na/K) Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-ATPase (Na/K) Antikörper

Spezifität: This antibody reacts to Sodium Potassium ATPase.

Aufreinigung: Ion exchange chromatography

Immunogen: ATPase, Sodium, Potassium [Canine Kidney]

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/100 to 1/500. ELISA: 1/1,000 to 1/6,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent).

Konzentration: 10.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu ATPase (Na/K)

Target: ATPase (Na/K)

Hintergrund: The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.Synonyms: ATPase Sodium/Potasium, Sodium pump 1, Sodium/potassium dependent ATPase