L1/ORF2 Antikörper

Produktnummer ABIN401095

Der Huhn Polyklonal anti-L1/ORF2 Antikörper wird verwendet zum Nachweis von L1/ORF2 in Proben von Schimpanse und Human. Er wurde validiert für EIA und WB.

Kurzübersicht für L1/ORF2 Antikörper (ABIN401095)

Target: L1/ORF2

Reaktivität: Schimpanse, Human

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Enzyme Immunoassay (EIA), Western Blotting (WB)

Produktdetails

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human.

Aufreinigung: IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer.

Immunogen: Two Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10000. Western Blot: 1/2000 - 1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Antigendetails

Target: L1/ORF2

Andere Bezeichnung: L1 / ORF2

Hintergrund: The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retrotransposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.Synonyms: LINE-1