Protein G Antikörper (HRP)

Produktnummer ABIN401071

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Protein G in DB, WB, EIA und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Streptococcus.

Kurzübersicht für Protein G Antikörper (HRP) (ABIN401071)

Target: Protein G

Reaktivität: Streptococcus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Protein G Antikörper ist konjugiert mit HRP

Applikation: Dot Blot (DB), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Protein G Antikörper (HRP)

Aufreinigung: Ion exchange chromatography

Immunogen: Protein G [Streptococcus species]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot. Dot Blot. ELISA: 1/20000 to 1/100000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent). For extended storage reconstitute product with 50 % glycerol.

Konzentration: 10.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Protein G

Target: Protein G

Hintergrund: Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.