UBA1 Antikörper

Produktnummer ABIN401028

Der Kaninchen Polyklonal anti-UBA1 Antikörper (ABIN401028) detektiert spezifisch UBA1 in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Hund und Kaninchen.

Kurzübersicht für UBA1 Antikörper (ABIN401028)

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reaktivität: Human, Ratte, Maus, Hund, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-UBA1 Antikörper

Spezifität: This antibody is directed against Ubiquitin Activating Enzyme E1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Rabbit, Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Purified

Immunogen: Recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute with 0.1 mL of deionized water or equivalent.

Konzentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Andere Bezeichnung: UBE1 / UBA1

Hintergrund: Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitinprotein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a highenergy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 Molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.Synonyms: A1S9T, Protein A1S9, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1

Gen-ID: 7317

NCBI Accession: NP_003325

UniProt: P22314