MHV NSP9 Antikörper

Produktnummer ABIN400814

Der Maus Monoklonal anti-MHV NSP9 Antikörper wird verwendet zum Nachweis von MHV NSP9 in Proben von Maus. Er wurde validiert für WB und IF.

Kurzübersicht für MHV NSP9 Antikörper (ABIN400814)

Target: MHV NSP9

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 2C6-H1

Produktdetails

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Produktmerkmale: Synonyms: Nonstructural Protein 9, Mouse Hepatitis Virus Strain A59, Murine coronavirus

Aufreinigung: Protein A chromatography

Immunogen: E. coli derived fulllength MHV-A59 nsp9 protein. Remarks: This protein is part of the viral replicase polyprotein.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 1,000 (Expect a band approximately 13 kDa in size corresponding to matureMHV-A59 nsp9 in the appropriate cell lysate or extract). Immunoflourescence: 1: 1,000 (Vero-E6 cells were grown on glass slides followed byinfection with MHVA59 strain and fixation with PBS/3 % PFA. After washing andpermeabilization of the fixed cells, antibody incubation was performed in PBS/5 %FCS for30 min). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: MHV NSP9

Substanzklasse: Viral Protein

Hintergrund: The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.Synonyms: Mouse Hepatitis Virus Strain A59, Murine coronavirus, Nonstructural Protein 9

UniProt: P0C6X9