CCL5 Antikörper

Produktnummer ABIN400758

Der Maus Monoklonal anti-CCL5 Antikörper (Klon 8-PP-11) (ABIN400758) detektiert spezifisch CCL5 in Neut, EIA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CCL5 Antikörper (ABIN400758)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCL5 Antikörper ist unkonjugiert

Applikation: Neutralization (Neut), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 8-PP-11

Produktdetails anti-CCL5 Antikörper

Spezifität: Recognizes Human RANTES. The antibody effectively inhibits Chemotaxis of Human Monocytes, Human Lymphocytes and Rat peritoneal Macrophages induced by Human RANTES.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: C-C motif chemokine 5, D17S136E, SCYA5, Small-inducible cytokine A5, SIS-delta, EoCP,T-cell-specific protein RANTES, cell-specific protein P228, Eosinophil-chemotactic cytokine

Aufreinigung: Protein A Chromatography.

Immunogen: RANTES

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA: 2-4 μg/mL. Neutralization. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This CCL5 antibody was validated for use in Immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) Human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 40 % Glycerol without preservatives.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Andere Bezeichnung: RANTES / CCL5

Hintergrund: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gen-ID: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration