Granulin Antikörper (C-Term)

Produktnummer ABIN396952

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Granulin in WB, IHC, IP und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Granulin Antikörper (C-Term) (ABIN396952)

Target: Granulin (GRN)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Granulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Granulin Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Recognizes human PCDGF protein at ~64-88kD.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of human PCDGF (PC cell-derived growth factor, GP88, acrogranin, granulin precursor, epithelin precursor, or progranulin).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Human MDA-MB-231 breast adenocarcinoma, PC-3 prostate adenocarcinoma (bone metastatic site), HeLa cervical carcinoma, and HT1080 fibrosarcoma lysates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Granulin

Target: Granulin (GRN)

Andere Bezeichnung: GRN / Progranulin / Granulin

Hintergrund: Name/Gene ID: GRN
Family: Hormone

Synonyms: GRN, Acrogranin, CLN11, Epithelin precursor, GEP, gp88, Granulin, Granulin-epithelin, Granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin

Gen-ID: 2896

UniProt: P28799