PFKFB4 Antikörper (AA 266-296)

Produktnummer ABIN392786

Der Kaninchen Polyklonal Anti-PFKFB4-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, PFKFB4 in Proben von Human und Maus zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für PFKFB4 Antikörper (AA 266-296) (ABIN392786)

Target: PFKFB4 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 4 (PFKFB4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PFKFB4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB04059

Produktdetails anti-PFKFB4 Antikörper

Bindungsspezifität: AA 266-296

Homologie: Pr

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PFKFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-296 amino acids from the Central region of human PFKFB4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:100. IHC-P: 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-PFKFB4 Antikörper (ABIN392786)

Ros, Flöter, Kaymak, Da Costa, Houddane, Dubuis, Griffiths, Mitter, Walz, Blake, Behrens, Brindle, Zamboni, Rider, Schulze: "6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 is essential for p53-null cancer cells." in: Oncogene, Vol. 36, Issue 23, pp. 3287-3299, (2017) (PubMed).

Gómez, Manzano, Figueras, Viñals, Ventura, Rosa, Bartrons, Navarro-Sabaté: "Sertoli-secreted FGF-2 induces PFKFB4 isozyme expression in mouse spermatogenic cells by activation of the MEK/ERK/CREB pathway." in: American journal of physiology. Endocrinology and metabolism, Vol. 303, Issue 6, pp. E695-707, (2012) (PubMed).

Ros, Santos, Moco, Baenke, Kelly, Howell, Zamboni, Schulze: "Functional metabolic screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 as an important regulator of prostate cancer cell survival." in: Cancer discovery, Vol. 2, Issue 4, pp. 328-43, (2012) (PubMed).

Yalcin, Clem, Simmons, Lane, Nelson, Clem, Brock, Siow, Wattenberg, Telang, Chesney: "Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24223-32, (2009) (PubMed).

Blum, Jacob-Hirsch, Amariglio, Rechavi, Kloog: "Ras inhibition in glioblastoma down-regulates hypoxia-inducible factor-1alpha, causing glycolysis shutdown and cell death." in: Cancer research, Vol. 65, Issue 3, pp. 999-1006, (2005) (PubMed).

Details zu PFKFB4

Target: PFKFB4 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 4 (PFKFB4))

Andere Bezeichnung: PFKFB4

Hintergrund: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Molekulargewicht: 54040

Gen-ID: 5210

NCBI Accession: NP_004558

UniProt: Q16877