Dieses Anti-DGKB-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DGKB in WB und IHC (p). Geeignet für Human und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Klon
RB3877
Bindungsspezifität
AA 773-804, C-Term
Aufreinigung
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This DGKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-804 amino acids from the C-terminal region of human DGKB.
DGKB
Reaktivität: Maus
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 647
Applikationshinweise
WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit
6 months
Dorrell, Grompe, Pan, Zhong, Canaday, Shultz, Greiner, Wright, Streeter, Grompe: "Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers." in: Molecular and cellular endocrinology, Vol. 339, Issue 1-2, pp. 144-50, (2011) (PubMed).
Target
DGKB
(Diacylglycerol Kinase, beta 90kDa (DGKB))
Andere Bezeichnung
DGKB
Hintergrund
Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified