STK39 Antikörper (AA 346-376)

Produktnummer ABIN392497

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch STK39 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für STK39 Antikörper (AA 346-376) (ABIN392497)

Target: STK39 (serine threonine Kinase 39 (STK39))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STK39 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB03418

Produktdetails anti-STK39 Antikörper

Bindungsspezifität: AA 346-376

Homologie: Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-STK39 Antikörper (ABIN392497)

Yang, Cai, Zhou, Chen, Chen, Chen, Wang, Fang, Zhou: "STE20/SPS1-related proline/alanine-rich kinase is involved in plasticity of GABA signaling function in a mouse model of acquired epilepsy." in: PLoS ONE, Vol. 8, Issue 9, pp. e74614, (2013) (PubMed).

Wang, OConnell, McArdle, Wade, Dorff, Shah, Shi, Pan, Rampersaud, Shen, Kim, Subramanya, Steinle, Parsa, Ober, Welling, Chakravarti, Weder, Cooper, Mitchell, Shuldiner, Chang: "From the Cover: Whole-genome association study identifies STK39 as a hypertension susceptibility gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 1, pp. 226-31, (2009) (PubMed).

Smith, Smallwood, Altman, Liedtke: "PKCdelta acts upstream of SPAK in the activation of NKCC1 by hyperosmotic stress in human airway epithelial cells." in: The Journal of biological chemistry, Vol. 283, Issue 32, pp. 22147-56, (2008) (PubMed).

Liedtke, Wang, Smallwood: "Role for protein phosphatase 2A in the regulation of Calu-3 epithelial Na+-K+-2Cl-, type 1 co-transport function." in: The Journal of biological chemistry, Vol. 280, Issue 27, pp. 25491-8, (2005) (PubMed).

Details zu STK39

Target: STK39 (serine threonine Kinase 39 (STK39))

Andere Bezeichnung: SPAK

Hintergrund: SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.

Molekulargewicht: 59474

Gen-ID: 27347

NCBI Accession: NP_037365

UniProt: Q9UEW8