BRSK2 Antikörper (C-Term)

Produktnummer ABIN391285

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch BRSK2 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für BRSK2 Antikörper (C-Term) (ABIN391285)

Target: BRSK2 (BR serine/threonine Kinase 2 (BRSK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRSK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB5548

Produktdetails anti-BRSK2 Antikörper

Bindungsspezifität: AA 706-736, C-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This STK29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-736 amino acids from the C-terminal region of human STK29.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu BRSK2

Target: BRSK2 (BR serine/threonine Kinase 2 (BRSK2))

Andere Bezeichnung: STK29

Hintergrund: BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C, and CDC25B in an in vitro assay, but a kinase-dead mutant does not. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.

Molekulargewicht: 81633

Gen-ID: 9024

NCBI Accession: NP_001243556, NP_001243558, NP_001243559, NP_003948

UniProt: Q8IWQ3