TAO Kinase 1 (TAOK1) (AA 620-650) Antikörper

Produktnummer ABIN391121

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für TAO Kinase 1 (TAOK1) (AA 620-650) Antikörper (ABIN391121)

Target: TAO Kinase 1 (TAOK1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB9765

Produktdetails

Bindungsspezifität: AA 620-650

Homologie: X, M, Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This TAOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 620-650 amino acids from the Central region of human TAOK1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: TAO Kinase 1 (TAOK1)

Andere Bezeichnung: TAOK1

Hintergrund: TAOK1 is an upstream activator of Mark. TAOK1 phosphorylated Mark on a threonine within its activation loop. In brain, TAOK1 also phosphorylated a fraction of Mark on a nearby serine, and this phosphorylation inhibited Mark activity. In cells, TAOK1 activity enhanced microtubule dynamics through activation of Mark and led to phosphorylation and detachment of microtubule-associated proteins from microtubules. TAOK1 also activated JNK in vitro. Overexpression of TAOK1 in a human nonsmall cell lung carcinoma cell line induced apoptotic morphologic changes, including cell contraction, membrane blebbing, and apoptotic body formation. Apoptotic stimuli increased the catalytic activity of endogenous TAOK1 and JNK, and dominant-negative JNK or JNK inhibition blocked the apoptotic morphologic responses to TAOK1. TAOK1 also stimulated cleavage and activation of ROCK1 by caspases, leading to cell contraction and membrane blebbing. TAOK1 was itself a substrate for caspase-3. TAOK1 is indeed involved in the execution phase of apoptosis.

Molekulargewicht: 116070

Gen-ID: 57551

NCBI Accession: NP_065842, NP_079418

UniProt: Q7L7X3