This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This PRPS1/PRPS2/PRPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the N-terminal region of human PRPS1/PRPS2/PRPS3.
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
WB: 1:1000. WB: 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit
6 months
Funes, Quintero, Henderson, Martinez, Qureshi, Westwood, Clements, Bourboulia, Pedley, Moncada, Boshoff: "Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 15, pp. 6223-8, (2007) (PubMed).
Target
PRPS1,PRPS2,PRPS3
Andere Bezeichnung
PRPS1/PRPS2/PRPS3
Hintergrund
Phosphoribosylpyrophosphate synthetase (PRPS, EC 2.7.6.1) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine, pyrimidine, and pyridine biosynthesis. By PCR of human lymphoblast mRNA using primers based on the cDNA sequence of rat PRS I (Prps1), Roessler et al. (1990) isolated a partial human PRPS1 cDNA. They used this partial cDNA to screen lymphoblast cDNA libraries and isolated additional cDNAs corresponding to the entire PRPS1 coding region. The deduced PRPS1 protein has 318 amino acids.