Ubiquilin 1 Antikörper (AA 296-326)

Produktnummer ABIN388966

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Ubiquilin 1 in WB, IHC (p) und IF. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Ubiquilin 1 Antikörper (AA 296-326) (ABIN388966)

Target: Ubiquilin 1 (UBQLN1)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ubiquilin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Klon: RB4554

Produktdetails anti-Ubiquilin 1 Antikörper

Bindungsspezifität: AA 296-326

Homologie: Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Ubiquilin1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-326 amino acids from the Central region of human Ubiquilin1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:100. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-Ubiquilin 1 Antikörper (ABIN388966)

Chen, Wang, Jiang, Zheng, Justice, Wang, Ran, Li, Huo, Zhang, Li, Lu, Wang, Zheng, Long, Yang: "APP modulates KCC2 expression and function in hippocampal GABAergic inhibition." in: eLife, Vol. 6, (2018) (PubMed).

Bieniek, Murray, Rutherford, Castanedes-Casey, DeJesus-Hernandez, Liesinger, Baker, Boylan, Rademakers, Dickson: "Tau pathology in frontotemporal lobar degeneration with C9ORF72 hexanucleotide repeat expansion." in: Acta neuropathologica, Vol. 125, Issue 2, pp. 289-302, (2013) (PubMed).

Bao, Zhang, Zheng, Xu, Yan: "UBQLN1 interacts with SPEM1 and participates in spermiogenesis." in: Molecular and cellular endocrinology, Vol. 327, Issue 1-2, pp. 89-97, (2010) (PubMed).

Details zu Ubiquilin 1

Target: Ubiquilin 1 (UBQLN1)

Andere Bezeichnung: Ubiquilin1

Hintergrund: Ubiquilin 1 (UBQLN1), also known as DA41, was isolated from an adult rat lung cDNA library, and encodes a cellular protein that associates with DAN.1 DAN expression is reduced in rat fibroblast 3Y1 cells transformed with mouse sarcoma virus and in rodent fibroblasts transformed with a variety of oncogenes. The DAN-DA41 interaction is mediated through the N-terminal domain and a cysteine-knot region of DAN. Human DA41 encodes a 589-amino acid protein with 86 % amino acid sequence identity with rat protein.2 DA41 expression is regulated in a cell cycle-dependent manner. PLIC1 and PLIC2 (UBQLN2) are homologs of the mouse Plics (proteins linking integrin-associated protein (IAP) and cytoskeleton) and the yeast Dsk2 protein. PLIC1, also called UBQLN1, shares 72 % amino acid identity with PLIC2,3 Two motifs are conserved in the mammalian PLICs and yeast Dsk2, an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Unlike ubiquitin, the UBL domain of the PLICs does not have a diglycine motif in its C terminus. The UBA domain is present in multiple enzyme classes of the ubiquitination machinery. Human PLICs associate with both proteasomes and ubiquitin ligases in large complexes. Overexpression of PLICs impairs the in vivo degradation of 2 unrelated ubiquitin-dependent proteasome substrates, p53 and I-kappa-B-alpha (NFKBIA), but not a ubiquitin-independent substrate. PLICs may link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. The DA41 gene maps to chromosome 9q21.2-q21.3, a position overlapping a candidate tumor suppressor locus for bladder cancer.2

Molekulargewicht: 62519

Gen-ID: 29979

NCBI Accession: NP_038466, NP_444295

UniProt: Q9UMX0